Review



brightvision anti rabbit ap  (Vector Laboratories)


Bioz Verified Symbol Vector Laboratories is a verified supplier
Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Vector Laboratories brightvision anti rabbit ap
    Brightvision Anti Rabbit Ap, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1588 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+blue+substrate+kit/Vector+Blue+Alkaline+Phosphatase+(Blue+AP)+Substrate+Kit/10__22203_slash_ecm__v033a11-129-57-73
    Average 96 stars, based on 1588 article reviews
    brightvision anti rabbit ap - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Activity Assay:

    Article Title: A proprietary lipidosterolic extract of Serenoa repens promotes hair growth through mechanisms that extend beyond 5-alpha reductase inhibition: Insights from human hair follicle organ culture.
    Article Snippet: .. Alkaline phosphatase activity in situ was detected using the Vector Blue Substrate Kit and alkaline phosphatase (SK5300, Biozol) according to the manufacturer's instructions, with levamisole as a negative control [39, 53]. ..

    Article Title: Developmental rewiring of the NGAL/CUC/KLU network associated with pleiotropic roles of NGAL genes.
    Article Snippet: .. CUC2 transcripts are detected with Vector Blue Substrate Kit (AP activity) and CUC3 with Alexa FluorTM 488 Tyramide Reagent (POD activity). ..

    Article Title: Developmental rewiring of the NGAL/CUC/KLU network associated with pleiotropic roles of NGAL genes
    Article Snippet: .. CUC2 transcripts are detected with Vector Blue Substrate Kit (AP activity) and CUC3 with Alexa FluorTM 488 Tyramide Reagent (POD activity). ..

    In Situ:

    Article Title: A proprietary lipidosterolic extract of Serenoa repens promotes hair growth through mechanisms that extend beyond 5-alpha reductase inhibition: Insights from human hair follicle organ culture.
    Article Snippet: .. Alkaline phosphatase activity in situ was detected using the Vector Blue Substrate Kit and alkaline phosphatase (SK5300, Biozol) according to the manufacturer's instructions, with levamisole as a negative control [39, 53]. ..

    Negative Control:

    Article Title: A proprietary lipidosterolic extract of Serenoa repens promotes hair growth through mechanisms that extend beyond 5-alpha reductase inhibition: Insights from human hair follicle organ culture.
    Article Snippet: .. Alkaline phosphatase activity in situ was detected using the Vector Blue Substrate Kit and alkaline phosphatase (SK5300, Biozol) according to the manufacturer's instructions, with levamisole as a negative control [39, 53]. ..

    Staining:

    Article Title: Derivation of embryonic stem cells across avian species.
    Article Snippet: .. To perform alkaline phosphatase staining, we used Vector Blue Substrate Kit (SK-5300, Vector Laboratories) and followed the manufacturer’s instruction. .. Chicken blastodermal cells were isolated from fertile eggs and cultured under various conditions in four-well chamber slides (Nunc Lab-Tek II Chamber Slide, Thermo Fisher Scientific) preseeded with mitotically inactivated mouse embryonic fibroblasts.

    Article Title: Derivation of embryonic stem cells across avian species
    Article Snippet: .. To perform alkaline phosphatase staining, we used Vector Blue Substrate Kit (SK-5300, Vector Laboratories) and followed the manufacturer’s instruction. ..

    Incubation:

    Article Title: Effects of neuromelanin buildup in rodent nigral dopamine neurons: implications for sex-biased vulnerability in Parkinson's disease.
    Article Snippet: Sections were then incubated with a blocking solution made with 5% normal goat serum and 0.3% Triton X-100 (Sigma) in a PB solution for 1 h. Free-floating sections were incubated with the primary anti-TH antibody (1:800, Novus Cat# NB 300–108, RRID:AB_350436), diluted in PB containing 0.3% Triton X-100 overnight at 4 ◦C. .. After three rinses, sections were incubated with a biotinylated secondary antibody (Millipore Cat# AP132B, RRID:AB_92488) followed by the incubation with extravidinperoxidase (1:1000, Sigma-Aldrich Cat# e2886, RRID:AB_2620165), and visualized by Vector Blue substrate Kit (Vector Laboratories Cat# SK-4700, RRID:AB_2314425). .. Slides were then dehydrated in a series of alcohols (50%, 70%, 95%, 100%), cleared in xylenes, and coverslipped with Entellan mounting medium (Sigma).



    Similar Products

    brightvision anti rabbit ap  (Vector Laboratories)
    96
    Vector Laboratories brightvision anti rabbit ap
    Brightvision Anti Rabbit Ap, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+blue+substrate+kit/Vector+Blue+Alkaline+Phosphatase+(Blue+AP)+Substrate+Kit/10__22203_slash_ecm__v033a11-129-57-73
    Average 96 stars, based on 1 article reviews
    brightvision anti rabbit ap - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    vector blue  (Vector Laboratories)
    96
    Vector Laboratories vector blue
    Vector Blue, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+blue+substrate+kit/Vector+Blue+Alkaline+Phosphatase+(Blue+AP)+Substrate+Kit/10__22203_slash_ecm__v033a11-129-70-73
    Average 96 stars, based on 1 article reviews
    vector blue - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    vector blue alkaline phosphatase (blue ap) substrate kit  (Vector Laboratories)
    96
    Vector Laboratories vector blue alkaline phosphatase (blue ap) substrate kit
    Vector Blue Alkaline Phosphatase (Blue Ap) Substrate Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+blue+substrate+kit/Vector+Blue+Alkaline+Phosphatase+(Blue+AP)+Substrate+Kit/custom%40sk-5300%4010%2E22203%2Fecm%2Ev033a11
    Average 96 stars, based on 1 article reviews
    vector blue alkaline phosphatase (blue ap) substrate kit - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    vector blue substrate kit  (Vector Laboratories)
    96
    Vector Laboratories vector blue substrate kit
    Vector Blue Substrate Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+blue+substrate+kit/Vector+Blue+Alkaline+Phosphatase+(Blue+AP)+Substrate+Kit/pm41126502-103-9-9
    Average 96 stars, based on 1 article reviews
    vector blue substrate kit - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    blue alkaline phosphatase substrate vector blue ap substrate kit vector laboratories 150 seconds at rt  (Vector Laboratories)
    96
    Vector Laboratories blue alkaline phosphatase substrate vector blue ap substrate kit vector laboratories 150 seconds at rt
    Blue Alkaline Phosphatase Substrate Vector Blue Ap Substrate Kit Vector Laboratories 150 Seconds At Rt, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+blue+substrate+kit/Vector+Blue+Alkaline+Phosphatase+(Blue+AP)+Substrate+Kit/us12582675-585-36-40
    Average 96 stars, based on 1 article reviews
    blue alkaline phosphatase substrate vector blue ap substrate kit vector laboratories 150 seconds at rt - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    blue alkaline phosphatase substrate kit  (Vector Laboratories)
    96
    Vector Laboratories blue alkaline phosphatase substrate kit
    Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
    Blue Alkaline Phosphatase Substrate Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+blue+substrate+kit/Vector+Blue+Alkaline+Phosphatase+(Blue+AP)+Substrate+Kit/bio_rxiv__64898__2026__03__17__712482-125-9-14
    Average 96 stars, based on 1 article reviews
    blue alkaline phosphatase substrate kit - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    vector blue substrate  (Vector Laboratories)
    96
    Vector Laboratories vector blue substrate
    Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
    Vector Blue Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+blue+substrate+kit/Vector+Blue+Alkaline+Phosphatase+(Blue+AP)+Substrate+Kit/pm41720824-220-6-10
    Average 96 stars, based on 1 article reviews
    vector blue substrate - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline phosphatase-stained colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.

    Journal: bioRxiv

    Article Title: Replicable generation of rhesus macaque iPSCs for in vitro modeling of genetic frontotemporal dementia

    doi: 10.64898/2026.03.17.712482

    Figure Lengend Snippet: Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline phosphatase-stained colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.

    Article Snippet: After 5 days, colonies were stained with a Vector Blue Alkaline Phosphatase Substrate kit (Vector Laboratories, cat. no. SK-5300) as described in .

    Techniques: Cloning, Staining, Cell Culture

    Characterization of RhiPSC line W6 clone 5.07. (A) Amplicon sequencing of MAPT R406W with wild-type C allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P16, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid EM2K was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 94.5% positive for SOX2, 99.2% positive for OCT4, and 95% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P12. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.

    Journal: bioRxiv

    Article Title: Replicable generation of rhesus macaque iPSCs for in vitro modeling of genetic frontotemporal dementia

    doi: 10.64898/2026.03.17.712482

    Figure Lengend Snippet: Characterization of RhiPSC line W6 clone 5.07. (A) Amplicon sequencing of MAPT R406W with wild-type C allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P16, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid EM2K was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 94.5% positive for SOX2, 99.2% positive for OCT4, and 95% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P12. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.

    Article Snippet: After 5 days, colonies were stained with a Vector Blue Alkaline Phosphatase Substrate kit (Vector Laboratories, cat. no. SK-5300) as described in .

    Techniques: Amplification, Sequencing, Staining, Reverse Transcription Polymerase Chain Reaction, Control, Plasmid Preparation, Positive Control, Immunofluorescence, Flow Cytometry, Virus

    Characterization of RhiPSC line M6 clone 16.02. (A) Amplicon sequencing of MAPT R406W with mutant T allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P18, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid EM2K was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 93.1% positive for SOX2, 98% positive for OCT4, and 93.3% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P15. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.

    Journal: bioRxiv

    Article Title: Replicable generation of rhesus macaque iPSCs for in vitro modeling of genetic frontotemporal dementia

    doi: 10.64898/2026.03.17.712482

    Figure Lengend Snippet: Characterization of RhiPSC line M6 clone 16.02. (A) Amplicon sequencing of MAPT R406W with mutant T allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P18, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid EM2K was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 93.1% positive for SOX2, 98% positive for OCT4, and 93.3% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P15. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.

    Article Snippet: After 5 days, colonies were stained with a Vector Blue Alkaline Phosphatase Substrate kit (Vector Laboratories, cat. no. SK-5300) as described in .

    Techniques: Amplification, Sequencing, Mutagenesis, Staining, Reverse Transcription Polymerase Chain Reaction, Control, Plasmid Preparation, Positive Control, Immunofluorescence, Flow Cytometry, Virus